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econopac 10 dg desalting columns  (Bio-Rad)


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    Structured Review

    Bio-Rad econopac 10 dg desalting columns
    Econopac 10 Dg Desalting Columns, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 718 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/econopac+10+dg+desalting+column/Econo-Pac+10DG+Desalting+Columns/pm41500287-116-21-26
    Average 96 stars, based on 718 article reviews
    econopac 10 dg desalting columns - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Purification:

    Article Title: Peptides and methods for producing them
    Article Snippet: Briefly, the cell-free lysates were each applied to a column of Ni-NTA agarose (Qiagen), washed with wash buffer [50 mM NaH2PO4(pH 8.0), 300 mM NaCl, 20 mM imidazole], and the proteins eluted from the column with elution buffer [50 mM NaH2PO4(pH 8.0), 300 mM NaCl, 250 mM imidazole]. .. Column fractions containing purified proteins were pooled, buffer-exchanged to 10 mM Tris-HCl (pH 8.0) for the SpaA (GG00442), SpaC (GG00444), SpaD (GG02370), SpaE (GG02371), and SpaF (GG02372) proteins and to 50 mM sodium acetate (pH 5.1) for the SpaB (GG00443) protein using a BioRad EconoPac 10 DG desalting column, and concentrated using a 30 kDa Microsep filter (Pall Life Sciences). ..

    Filtration:

    Article Title: In vitro reconstitution and characterisation of the oxidative D-xylose pathway for production of organic acids and alcohols.
    Article Snippet: .. The fraction containing Ec AldA were pooled and the buffer was changed to 50 mM Tris–HCl buffer, pH 7.5, 100 mM NaCl by gel filtration using an EconoPac 10 DG desalting column (Bio-Rad). ..

    Article Title: In vitro reconstitution and characterisation of the oxidative d -xylose pathway for production of organic acids and alcohols
    Article Snippet: .. The fraction containing Ec AldA were pooled and the buffer was changed to 50 mM Tris–HCl buffer, pH 7.5, 100 mM NaCl by gel filtration using an EconoPac 10 DG desalting column (Bio-Rad). ..

    SDS Page:

    Article Title: Characterization of the SpaCBA Pilus Fibers in the Probiotic Lactobacillus rhamnosus GG
    Article Snippet: .. The Ab-containing fractions judged to be pure by SDS-PAGE analysis were pooled, buffer exchanged with coupling buffer (0.2 M NaHCO 3 –0.5 M NaCl, pH 8.3) by using an EconoPac 10 DG desalting column (Bio-Rad), and then passed through a 0.45-μm-pore-size membrane filter. .. These affinity-purified polyclonal Ab (∼3.75 mg) then were coupled covalently to the resin in a 1-ml Hi-Trap N-hydroxysuccinimide (NHS)-activated column (GE Healthcare Bio-Sciences, Uppsala, Sweden) by following the manufacturer's protocol.

    Membrane:

    Article Title: Characterization of the SpaCBA Pilus Fibers in the Probiotic Lactobacillus rhamnosus GG
    Article Snippet: .. The Ab-containing fractions judged to be pure by SDS-PAGE analysis were pooled, buffer exchanged with coupling buffer (0.2 M NaHCO 3 –0.5 M NaCl, pH 8.3) by using an EconoPac 10 DG desalting column (Bio-Rad), and then passed through a 0.45-μm-pore-size membrane filter. .. These affinity-purified polyclonal Ab (∼3.75 mg) then were coupled covalently to the resin in a 1-ml Hi-Trap N-hydroxysuccinimide (NHS)-activated column (GE Healthcare Bio-Sciences, Uppsala, Sweden) by following the manufacturer's protocol.



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